Training

Seminars and Presentations

  • Mild Elution of a Mouse Mab from ProSep
    Millipore ProSepA User Group
    September 30, 2008
  • Chromatography Forum of the Delaware Valley
    Oct 14, 2008

Chromatography Training

  • Techniques in Molecular and Cellular Biology,
    Uniformed Services University of the Health Sciences, Bethesda, MD, March, 2009.
  • Selected Topics in Bioengineering: Biotechnology and Bioproduction (BIOE468B) University of Maryland, College Park, MD January, 2009.
  • Introduction to Biochromatography
    Young Scholars Program, University of Maryland, College Park
    July 2008, 2009
  • Downstream Processing & Protein Purification Workshop
    University of Maryland, College Park, MD
    June 2008, 2009

Biochromatography Course Flier

Recent Publications & Citations

  • Lees, A, A. Topping, A. Razzaq, K. Reiter, and A. Lopez-Acosta. Purifying a Recalcitrant Therapeutic Recombinant Protein with a Mixed-Mode Chromatography Sorbent. Bioprocessing Int., Feb 2009, pps 42-49.
  • Lees, A. and M. Burkhardt. Improving protein separations with mixed mode chromatography. LCGC Magazine, Jan. 2009.
  • Chattopadhyay G, Q. Chen, J. Colino, A. Lees, CM Snapper. Intact bacteria inhibit the induction of humoral immune responses to bacterial-derived and heterologous soluble T cell-dependent antigens. J Immunol. 182:2011, 2009.
  • Colino J, G. Chattopadhyay, G. Sen, Q. Chen, A. Lees, D.H. Canaday, A. Rubtsov, R. Torres and C.M. Snapper. Parameters underlying distinct T cell-dependent polysaccharide-specific IgG responses to an intact gram-positive bacterium versus a soluble conjugate vaccine. J Immunol. 183:1551, 2009.
  • Lees, A., V. Puvanesarajah and C.E. Frasch. Conjugation Chemistry. Pps 163-174 Pneumocococcal Vaccines: The Impact of Conjugate Vaccines. Eds G.R. Siber, K.P. Klugman and P.H. Makela. ASM Press, Washington DC. 2008.
  • Datta, K, A. Lees, LA. Pirofski. Therapeutic Efficacy of a Conjugate Vaccine containing a Peptide Mimotope of the Cryptococcal Capsular Polysaccharide Glucuronoxylomannan (GXM). Clin. Vaccine Immunol. 8:1176, 2008.
  • Petrushina I, Ghochikyan A, Mkrtichyan M, Mamikonyan G, Movsesyan N, Ajdari R, Vasilevko V, Karapetyan A, Lees A, Agadjanyan MG, Cribbs DH. Mannan-Abeta28 conjugate prevents Abeta-plaque deposition, but increases microhemorrhages in the brains of vaccinated Tg2576 (APPsw) mice. J Neuroinflam. 5:42, 2008.
  • Burkhardt, M., C. Sei, A. Lees, J. Mond. A rapid opsonic assay for measuring killing of bioluminescent Stapylocccous epidermidis. Hybridoma, 27:487, 2008.
  • Eluting Possibilities with Mixed-Mode Chromatography: Mixed-mode chromatography sorbents and custom ligands aim to optimize protein purification. Maribel Rios. Pharmaceutical Technology May 2, 2007.
  • Purification of soluble CD14 fusion proteins and use in an electrochemiluminescent assay for lipopolysaccharide binding. Burkhardt, M., A. Lopezacosta, K. Reiter, V. Lopez and A. Lees. Protein Exp. Purif., 51:96, 2007.
  • Aβ-immunotherapy for Alzheimer’s disease using mannan-amyloid-β peptide immunoconjugates. Ghochikyan A, Petrushina I, Lees A, Vasilevko V, Movsesyan N, Karapetyan A, Agadjanyan MG, Cribbs DH. DNA Cell Biol. 25:571, 2006